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Related Experiment Videos

Changes in CD45 isoform expression after influenza vaccination

J E McElhaney1, M J Pinkoski, G S Meneilly

  • 1Division of Geriatric Medicine, University of Alberta, Edmonton, Canada.

Mechanisms of Ageing and Development
|June 1, 1993
PubMed
Summary

Elderly individuals show similar T lymphocyte responses to influenza vaccination as young adults, with changes in CD45 isoforms suggesting memory exists in CD45RO- cells. This indicates effective immune memory despite aging.

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Area of Science:

  • Immunology
  • Gerontology

Background:

  • CD45 isoforms (CD45RA and CD45RO) are critical markers for T lymphocyte differentiation and memory.
  • Aging is associated with altered immune cell phenotypes, potentially impacting vaccine responses.

Purpose of the Study:

  • To investigate changes in CD45 isoform expression on T lymphocytes post-influenza vaccination in young versus elderly adults.
  • To determine if influenza vaccination elicits similar T lymphocyte responses across different age groups.

Main Methods:

  • Comparison of T lymphocyte CD45 isoform profiles (CD45RA, CD45RO) in young and elderly adults before and after influenza vaccination.
  • Analysis of peripheral blood mononuclear cells (PBMCs) using flow cytometry with fluorescent monoclonal antibodies.
  • In vitro stimulation of PBMCs with influenza virus to assess recall responses.

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Main Results:

  • Elderly individuals exhibited a higher proportion of CD45RO+RA- and lower CD45RA+RO- T cells compared to young adults.
  • Both groups demonstrated a progressive increase in CD45RA+ T lymphocytes post-vaccination.
  • In vitro viral stimulation led to a decrease in CD45RA+RO- and an increase in CD45RA-RO- cells at 6 weeks post-vaccination.
  • The elderly group displayed comparable changes in cell phenotypes to the young group despite age-related declines in CD45RA+ cells.

Conclusions:

  • Influenza vaccination induces similar T lymphocyte phenotype changes in both young and elderly individuals.
  • The findings suggest that influenza-specific memory may reside within the CD45RO- T cell population.
  • Immune memory responses to influenza split-virus vaccine are preserved in the elderly, despite age-related immune alterations.