Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and function of endoglucanase V

G J Davies1, G G Dodson, R E Hubbard

  • 1Department of Chemistry, University of York, Heslington, UK.

Nature
|September 23, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combinatorial assembly and design of enzymes.

Science (New York, N.Y.)·2023
Same author

Analysis of transition state mimicry by tight binding aminothiazoline inhibitors provides insight into catalysis by human <i>O</i>-GlcNAcase.

Chemical science·2018
Same author

Structural and mechanistic insights into a Bacteroides vulgatus retaining N-acetyl-β-galactosaminidase that uses neighbouring group participation.

Chemical communications (Cambridge, England)·2016
Same author

Does inhalation allergy to pig exist?

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2016
Same author

Functional Examination of the Shoulder Girdle.

The Physician and sportsmedicine·2016
Same author

Letters.

The Physician and sportsmedicine·2016

Researchers discovered the structure of endoglucanase V, a novel cellulase enzyme. This enzyme, crucial for breaking down plant cellulose, exhibits a unique structure distinct from other known cellulases.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Enzymology

Background:

  • Cellulose is the most abundant organic polymer on Earth, primarily found in plant cell walls.
  • Cellulases are enzymes produced by microorganisms to hydrolyze beta-(1,4) glycosidic bonds in cellulose, enabling its use as a nutrient source.
  • Existing knowledge of cellulase structures is limited to members of two out of seven distinct families.

Purpose of the Study:

  • To determine the three-dimensional structure of endoglucanase V, a cellulase from a previously uncharacterized family.
  • To compare the structure of endoglucanase V with other known cellulases and related proteins.
  • To investigate the structural basis for the enzyme's catalytic activity and its potential functional relationships.

Main Methods:

  • X-ray crystallography was employed to elucidate the three-dimensional structure of endoglucanase V.

Related Experiment Videos

  • Sequence analysis was performed to determine the enzyme's classification and relationship to other proteins.
  • Structural comparisons were made with databases of known protein structures.

    • Main Results:

    • The structure of endoglucanase V was determined, revealing it belongs to a distinct structural family of cellulases.
    • Endoglucanase V is structurally dissimilar to previously characterized cellulases but shares similarities with a plant defense protein.
    • The active site of endoglucanase V shows a resemblance to that of lysozyme, despite overall structural differences.

    Conclusions:

    • Endoglucanase V represents a novel structural class of cellulases, expanding our understanding of enzyme diversity.
    • The structural similarity to a plant defense protein suggests potential roles beyond cellulose degradation.
    • The lysozyme-like active site points to conserved catalytic mechanisms in glycoside hydrolases.