Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ciliary neurotrophic factor is an endogenous pyrogen

L Shapiro1, X X Zhang, R G Rupp

  • 1Department of Medicine, Tuffs University School of Medicine, Boston, MA.

Proceedings of the National Academy of Sciences of the United States of America
|September 15, 1993
PubMed
Summary

Ciliary neurotrophic factor (CNTF) directly causes fever in rabbits by acting on the hypothalamus. This pyrogenic effect, distinct from endotoxins, involves signaling pathways similar to interleukin 6.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical, hematologic, and immunologic effects of interleukin-10 in humans.

Journal of clinical immunology·1996
Same author

Protein metabolism in rheumatoid arthritis and aging. Effects of muscle strength training and tumor necrosis factor alpha.

Arthritis and rheumatism·1996
Same author

Effect of endotoxin in IL-1 beta-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)·1996
Same author

Modulation of osteoclast-activating factor activity of multiple myeloma bone marrow cells by different interleukin-1 inhibitors.

Experimental hematology·1996
Same author

Thermoregulation and the pathogenesis of fever.

Infectious disease clinics of North America·1996
Same author

Three distinct promoters direct transcription of different 5' untranslated regions of the human interleukin 1 type I receptor: a possible mechanism for control of translation.

Cytokine·1996

Area of Science:

  • Neuroscience
  • Immunology
  • Physiology

Background:

  • Fever results from endogenous pyrogens elevating the hypothalamic thermoregulatory set point.
  • Ciliary neurotrophic factor (CNTF) is known for its role in neuronal support.
  • The pyrogenic potential of CNTF has not been previously established.

Purpose of the Study:

  • To investigate the intrinsic pyrogenic activity of Ciliary neurotrophic factor (CNTF).
  • To determine the mechanism and characteristics of CNTF-induced fever.
  • To explore the relationship between CNTF and other endogenous pyrogens.

Main Methods:

  • Intravenous injection of CNTF in rabbits.
  • Administration of indomethacin as a pretreatment.
  • Analysis of plasma for endogenous pyrogen activity post-injection.

Related Experiment Videos

  • In vitro assessment of CNTF's effect on interleukin 1, tumor necrosis factor, and interleukin 6 production.

    • Main Results:

    • CNTF induced a rapid, monophasic fever in rabbits, independent of endotoxins.
    • Indomethacin pretreatment blocked the fever response to CNTF.
    • Higher CNTF doses prolonged the fever, suggesting secondary pyrogenic activity.
    • Plasma transfer from CNTF-treated rabbits did not transfer pyrogenic activity; CNTF did not induce IL-1, TNF, or IL-6 in vitro.

    Conclusions:

    • CNTF possesses intrinsic pyrogenic activity, directly inducing fever.
    • CNTF-induced fever is mediated by prostaglandin synthesis and potentially involves central nervous system mechanisms.
    • CNTF shares the gp130 signal-transduction pathway with interleukin 6 for its pyrogenic effects.