Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor

K Tanaka1, J Davey, Y Imai

  • 1Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.

Molecular and Cellular Biology
|January 1, 1993
PubMed
Summary

The map3 gene in Schizosaccharomyces pombe is crucial for mating, acting as the M-factor receptor. Its defect causes sterility, highlighting pheromone signaling

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The experience and supportive care needs in people affected by ovarian cancer and their informal caregivers: a qualitative systematic review.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

Urethral end-to-end anastomosis in a 9-year-old cat after complete urethral rupture secondary to fall injury.

Australian veterinary journal·2025
Same author

Accurate measurement of the through-plane water content of proton-exchange membranes using neutron radiography.

Journal of applied physics·2022
Same author

'Careful goodbye at the door': is there role for antimicrobial stewardship interventions for antimicrobial therapy prescribed on hospital discharge?

BMC infectious diseases·2018
Same author

Does getting away with it count? An application of stafford and warr's reconceptualised model of deterrence to drink driving.

Accident; analysis and prevention·2017
Same author

Is there an observational effect? An exploratory study into speed cameras and self-reported offending behaviour.

Accident; analysis and prevention·2017

Area of Science:

  • * Molecular and Cellular Biology
  • * Yeast Genetics
  • * Reproductive Biology

Background:

  • * Mating-type determination and pheromone signaling are critical for sexual reproduction in many fungi.
  • * Fission yeast Schizosaccharomyces pombe exhibits distinct mating types (h+ and h-) and utilizes pheromones for conjugation.
  • * Previous studies identified genes involved in mating but the specific receptor for M-factor in S. pombe was not fully characterized.

Purpose of the Study:

  • * To identify and characterize the gene responsible for M-factor reception in Schizosaccharomyces pombe.
  • * To elucidate the role of the map3 gene in mating-type-specific sterility and pheromone response.
  • * To investigate the involvement of pheromone signaling in the initiation of meiosis.

Main Methods:

Related Experiment Videos

  • * Gene cloning by complementation of a sterile mutant.
  • * Nucleotide sequencing and analysis of the cloned gene.
  • * Expression analysis of the map3 gene under different conditions.
  • * Functional assays involving ectopic gene expression and response to purified M-factor.

Main Results:

  • * The map3 gene was cloned and found to encode a putative seven-transmembrane protein with homology to known mating pheromone receptors.
  • * map3 gene expression is specific to h+ cells and induced by nitrogen starvation.
  • * Defects in map3 abolish response to M-factor, while ectopic expression in h- cells confers M-factor responsiveness.
  • * The G-protein alpha-subunit gene (gpa1) is essential for map3 function.

Conclusions:

  • * The map3 gene encodes the M-factor receptor in Schizosaccharomyces pombe.
  • * M-factor receptor function is essential for initiating conjugation and subsequent meiotic processes.
  • * Pheromone signaling pathways are vital for sexual reproduction initiation in S. pombe.