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Related Experiment Videos

Structural requirements for simian virus 40 replication and virion maturation

R Schirmbeck1, A von der Weth, W Deppert

  • 1Abteilung Tumorvirologie, Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Hamburg, Germany.

Journal of Virology
|February 1, 1993
PubMed
Summary

Newly replicated simian virus 40 (SV40) DNA accumulates at the nuclear matrix, where it is directly packaged into new virions. Most progeny virions remain associated with the nuclear matrix until cell lysis.

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Area of Science:

  • Molecular Biology
  • Virology
  • Cell Biology

Background:

  • The nuclear matrix is crucial for simian virus 40 (SV40) DNA replication in vivo.
  • Replication complexes are anchored to the nuclear matrix.

Purpose of the Study:

  • To analyze the events from nuclear matrix-associated replication to SV40 virion encapsidation.
  • To understand the fate of newly replicated SV40 minichromosomes.

Main Methods:

  • Pulse-chase experiments were used to track SV40 minichromosomes.
  • Analysis of SV40 replication and virion assembly at the nuclear matrix.

Main Results:

  • Newly replicated SV40 minichromosomes accumulate at the nuclear matrix.
  • Direct encapsidation of SV40 virions occurs at the nuclear matrix.

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  • Progeny virions are released from the nuclear matrix, but the majority remain until cell lysis.

    • Conclusions:

    • The nuclear matrix serves as a platform for SV40 DNA replication, virion assembly, and storage.
    • SV40 virion release and distribution are linked to the nuclear matrix throughout infection.