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Updated: Sep 29, 2026

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
Published on: December 12, 2015
Estimating rapid changes in the rates of glucose production from glycogenolysis and recycling through lactate
Abstract:
In order to obtain independent estimates of changes in the rate of glucose production from glycogenolysis from the glucose carbon recycled throught lactate, previously validated nonsteady tracer methods are used. Changes in glycogenolysis are estimated by calculating the rate of appearance of a label ([6-3H]glucose) uniformly distributed through glycogen and simultaneously the rate of recycling of 14C is measured under nonsteady-state conditions. It is indicated that these results can be extended to the determination of absolute rates of glucose production from glycogen breakdown and gluconeogenetic precursors.
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