Androgen regulation of transferase II activity in the mouse kidney

Castration of adult male mice reduced the ability of the transferase factors of the kidney to stimulate amino acid incorporation by polysomes in vitro. The administration of testosterone propionate (TP) to the mice for 11 days greatly increased the activity of the transferase fraction. The changes occurred only in transferase II. The induction of the increase in transferase activity was evident 24 h after the injection of TP and required a lag period of at least 12 h. The concentration of pH 5 enzymes (protein) was slightly decreased by castration and was restored by TP administration. The radioactivity in the hot perchloric acid extract of the protein after amino acid incorporation was increased but the activity of the pH 5 enzyme fraction on amino incorporation was not significantly changed by androgen administration.