Related Experiment Videos
Effect of heparin on hemagglutination by pseudorabies virus
1Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Abstract:
Heparin inhibited hemagglutination (HA) by pseudorabies virus (PRV), but not HA by Akabane virus, bovine adenovirus type 7, Fukuoka virus, Getah virus, Japanese encephalitis virus, and parainfluenza virus type 3 belonging to the families Bunyaviridae, Adenoviridae, Rhabdoviridae, Togaviridae, Flavivi-idae, and Paramyxoviridae, respectively. The minimal inhibitory concentration of heparin required to inhibit 8 HA U of PRV ranged from 0.005 to 0.01 U/ml. Mouse erythrocytes failed to combine with the HA inhibitory factor of heparin. On the other hand, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by PRV. Virus-heparin complex formation could be observed by sedimenting heparin with the virus particles.
Insights
Heparin effectively inhibits hemagglutination (HA) by pseudorabies virus (PRV), but not by other tested viruses. This suggests a specific interaction between heparin and PRV, impacting viral agglutination capabilities.
Area of Science:
- Virology
- Biochemistry
Background:
- Hemagglutination (HA) is a common viral property.
- Heparin is a known anticoagulant with potential antiviral properties.
Purpose of the Study:
- To investigate the inhibitory effect of heparin on hemagglutination by various viruses.
- To determine the specificity of heparin's interaction with pseudorabies virus (PRV).
Main Methods:
- Hemagglutination inhibition assays were performed using heparin and multiple viruses.
- Minimal inhibitory concentrations of heparin were determined for PRV HA.
- Erythrocyte-heparin interactions and virus-heparin complex formation were analyzed.
Main Results:
- Heparin specifically inhibited hemagglutination by pseudorabies virus (PRV).
- Heparin did not inhibit HA by viruses from Bunyaviridae, Adenoviridae, Rhabdoviridae, Togaviridae, Flaviviridae, and Paramyxoviridae families.
- Virus-heparin complex formation was observed, indicating a direct interaction.
Conclusions:
- Heparin exhibits specific inhibitory activity against pseudorabies virus hemagglutination.
- The interaction between heparin and PRV is distinct from its interaction with other tested viral families.