Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatitis C in liver allografts

S N Thung1, K S Shim, Y S Shieh

  • 1Lillian and Henry M. Stratton-Hans Popper Department of Pathology, Mount Sinai School of Medicine, City University of New York, NY 10029.

Archives of Pathology & Laboratory Medicine
|February 1, 1993
PubMed
Summary

Hepatitis C virus (HCV) RNA detection in liver transplants reliably diagnoses HCV infection. This method is crucial for differentiating HCV from allograft rejection in transplant patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Iatrogenic scald burn: a consequence of institutional infection control measures.

Pediatrics·1996
Same author

Reconstitution of mammalian pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes: analysis of protein X involvement and interaction of homologous and heterologous dihydrolipoamide dehydrogenases.

The Biochemical journal·1996
Same author

Nonequilibrium gating and voltage dependence of the ClC-0 Cl- channel.

The Journal of general physiology·1996
Same author

Nosocomial infections with vancomycin-resistant Enterococcus faecium in liver transplant recipients: risk factors for acquisition and mortality.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·1996
Same author

Homodimeric architecture of a ClC-type chloride ion channel.

Nature·1996
Same author

Evolution of promoter sequences: elements of a canonical promoter for prespore genes of Dictyostelium.

Journal of molecular evolution·1996

Area of Science:

  • Hepatology
  • Transplant Surgery
  • Virology

Background:

  • Recurrent or intercurrent hepatitis C poses challenges in liver transplant recipients.
  • Distinguishing hepatitis C from allograft rejection is critical but difficult with standard tests.
  • Pre-existing or delayed anti-hepatitis C virus (HCV) antibody development complicates diagnosis.

Purpose of the Study:

  • To evaluate the utility of detecting HCV RNA in liver allograft biopsies for diagnosing hepatitis C post-transplant.
  • To assess the reliability of polymerase chain reaction (PCR) for HCV RNA detection in differentiating hepatitis C from allograft rejection.

Main Methods:

  • HCV RNA was detected using reverse double polymerase chain reaction (RT-PCR) in liver allograft biopsy specimens.
  • The study included nine patients with hepatitis of uncertain origin and three with cellular allograft rejection.

Related Experiment Videos

  • NS3 region sequences of HCV were analyzed.

    • Main Results:

    • HCV RNA was detected in seven out of nine liver allograft biopsy specimens.
    • HCV RNA was found in specimens collected 6 weeks to 20 months post-transplantation.
    • HCV RNA was absent in two patients, one of whom tested positive for anti-HCV antibodies.

    Conclusions:

    • Hepatitis C virus is a significant cause of hepatitis after liver transplantation.
    • Detecting HCV RNA in liver biopsy specimens via PCR is a reliable diagnostic method for hepatitis C in allografts.
    • HCV infection post-transplant can result from acquisition during transplantation or recurrence of a prior infection.