Cyclic adenosine 3',5'-monophosphate, a second messenger in interleukin-1 mediated K562 cytostasis

This study evaluates intracellular cAMP concentrations, in interleukin-1 (IL-1) mediated cytostasis of K562 cells. Dibutyryl-cAMP, forskolin, staurosporine and phorbol 12-myristate 13-acetate were all found to mimic the cytostatic action of IL-1 on K562 cells. IL-1 was found to elicit a dose-dependent rise in adenyl cyclase activity in isolated K562 membranes, as well as increasing cAMP concentrations in whole cells. cAMP concentrations were found to peak within 3 minutes of IL-1 stimulation. A 27-29% reduction in proliferative activity elicited by IL-1, forskolin and staurosporine, correlated with a 6 fold increase in adenyl cyclase activity, in each case.