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Detection of bovine leukemia virus RNA in serum using the polymerase chain reaction
H Poon1, E Jimenez, R M Jacobs
1Connaught Product Development Centre, Connaught Laboratories Limited, Willowdale, Ont., Canada.
Journal of Virological Methods
|January 1, 1993
Abstract:
A method was developed for detecting bovine leukemia virus (BLV) RNA in serum samples using a pair of primers from the BLV polymerase gene in the polymerase chain reaction (PCR). The PCR was able to detect 3800-7600 molecules of BLV RNA. At this level of sensitivity eleven pools of adult and one fetal bovine serum appeared free from BLV contamination.