Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD28- T lymphocytes. Antigenic and functional properties

M Azuma1, J H Phillips, L L Lanier

  • 1DNAX Research Institute of Molecular and Cellular Biology, Department of Immunology, Palo Alto, CA 94304.

Journal of Immunology (Baltimore, Md. : 1950)
|February 15, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term orthognathic surgical outcomes in Treacher Collins patients.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2015
Same author

Evidence for three types of human cytotoxic lymphocyte.

Immunology today·2014
Same author

Lymphokine-activated killer cell activity Characteristics of effector cells and their progenitors in blood and spleen.

Immunology today·2014
Same author

Interleukin-23 is sufficient to induce rapid de novo gut tumorigenesis, independent of carcinogens, through activation of innate lymphoid cells.

Mucosal immunology·2013
Same author

Paediatric craniofacial fibrous dysplasia: the Hospital for Sick Children experience and treatment philosophy.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2013
Same author

Weil's disease; a report of 23 cases.

The New England journal of medicine·2010

A subset of CD8+ T cells lacking CD28 expression exhibit potent cytotoxicity but impaired proliferation, suggesting distinct roles in immune responses. These CD28- T cells may represent a memory population with unique functions.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD8+ T cells are crucial for adaptive immunity.
  • CD28 is a co-stimulatory receptor involved in T cell activation.
  • A subset of CD8+ T cells lacks CD28 expression.

Purpose of the Study:

  • To investigate the functional characteristics of CD28- T cells.
  • To determine the potential role of CD28- T cells in immune responses.

Main Methods:

  • Flow cytometry to identify and sort CD28- and CD28+ T cells.
  • Assessment of cytotoxicity and proliferation assays.
  • Generation and analysis of T cell clones.

Main Results:

  • CD28- T cells are large granular lymphocytes expressing adhesion molecules but not acute activation markers.

Related Experiment Videos

  • Freshly isolated CD28- T cells exhibit potent cytotoxicity but reduced proliferation.
  • CD28- T cell clones are cytotoxic and anergic to T cell receptor stimulation, with variable CD28 re-expression.

    • Conclusions:

    • CD28- and CD28+ T cells likely play distinct roles in immune responses.
    • CD28- T cells may represent a memory population with cytotoxic functions.
    • The anergic phenotype of CD28- T cells suggests a regulatory or specialized effector function.