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Related Experiment Videos

Thymus-pituitary interactions during ageing

R G Goya1, M G Castro, P W Saphier

  • 1Department of Biochemistry and Physiology, University of Reading.

Age and Ageing
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

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Nucleoprotein complexes, like nucleohistone, can stimulate adrenocorticotropic hormone (ACTH) release from pituitary cells. However, this response diminishes with age, suggesting a decline in hormonal signaling in older rats.

Area of Science:

  • Endocrinology
  • Cell Biology
  • Molecular Biology

Background:

  • Homoeostatic thymus hormone (HTH), an H2A-H2B histone dimer, exhibits age-dependent adrenostimulatory activity.
  • Investigating the role of histones and related molecules in hormonal regulation is crucial for understanding aging processes.

Purpose of the Study:

  • To assess the adrenocorticotropin (ACTH)-releasing activity of histones and related substances on rat pituitary cells.
  • To compare the responsiveness of pituitary cells from young and mature rats to these substances.

Main Methods:

  • Utilized perifused pituitary cell cultures from young (2-4 months) and mature (16-18 months) rats.
  • Assayed for ACTH and corticotropin-releasing hormone (CRH) using immunoradiometric assays (IRMA).
  • Administered histones, protamines, histone fragment MB35, nucleohistone, and nucleoprotamine complexes.

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Main Results:

  • Histones and protamines caused assay interference, unlike MB35, nucleohistone, and nucleoprotamine.
  • Nucleohistone and nucleoprotamine complexes significantly stimulated ACTH release in cells from young rats.
  • A reduced, but still significant, ACTH response was observed in mature rat pituitary cells.

Conclusions:

  • Extracellular nucleoprotein complexes may function as hypophysiotropic signals regulating ACTH release.
  • Pituitary cell responsiveness to these signals decreases with donor age.
  • This age-related decline impacts the endocrine system's ability to respond to specific molecular cues.