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Evaluation of human kidney viability during cold storage
C M Helou1, A C Seguro, A dos S Rocha
1Laboratório de Pesquisa Básica, Nefrologia Faculdade de Medicina, Universidade de São Paulo, Brazil.
Renal Failure
|January 1, 1993
Summary
Cadaver kidneys show early injury signs in the thick ascending limb of Henle's loop within 20 hours of cold storage. However, proximal tubule function remains preserved for up to 70 hours, indicating potential viability for transplantation.
Area of Science:
- Nephrology
- Transplantation Biology
- Organ Preservation
Background:
- Assessing ischemic injury in cadaver kidneys is crucial for successful transplantation.
- Cold storage duration impacts kidney viability and function.
- Understanding cellular injury markers aids in predicting organ quality.
Purpose of the Study:
- To evaluate ischemic injury in human cadaver kidneys after cold storage.
- To compare injury markers in human kidneys with experimental rabbit models.
- To determine the functional preservation of kidney tubules during cold storage.
Main Methods:
- Measuring Na-K-ATPase activity in kidney tissues.
- Assessing "in vitro" para-aminohippuric acid (PAH) uptake.
- Comparing human kidney data with rabbit kidney models after varying cold storage periods.
Main Results:
- Human kidneys showed reduced Na-K-ATPase activity in the outer medulla within 20 hours of cold storage, indicating early thick ascending limb injury.
- PAH uptake, a marker of proximal tubule function, decreased significantly in rabbit kidneys after 72 hours.
- No significant injury progression was observed in the superficial cortex of human kidneys regarding PAH uptake and Na-K-ATPase activity up to 70 hours.
Conclusions:
- Early ischemic injury occurs in the thick ascending limb of Henle's loop in cadaver kidneys within 20 hours of cold storage.
- Proximal tubule function appears preserved in cadaver kidneys for up to 70 hours of cold storage.
- These findings suggest potential viability of cadaver kidneys for transplantation within this timeframe, with specific attention to early tubular injury.