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Modification of tryptophan 149 of inorganic pyrophosphatase from Escherichia coli
1Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University, Nagano-ken, Japan.
The International Journal of Biochemistry
|February 1, 1993
Abstract:
1. The inorganic pyrophosphatase from Escherichia coli was almost completely inactivated on chemical modification of Trp-149 with N-bromosuccinimide (NBS). 2. The presence of a complex of Mg2+ and a substrate analogue, iminodiphosphate (PNP), provided considerable protection against the inactivation, whereas Mg2+ or PNP alone afforded only slight protection.