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Expression of hepatitis A virus poly(U) polymerase in the periplasmic space of Escherichia coli
A J Wolstenholme1, J P Coates, W Douglass
1Department of Biochemistry, University of Bath, Claverton Down, U.K.
The Journal of General Virology
|March 1, 1993
Abstract:
A cDNA containing almost all of the hepatitis A virus (HAV) P3 sequences was expressed as a fusion protein with Protein A. A novel poly(U) polymerase activity was detected in the periplasmic space of Escherichia coli cells transformed with this plasmid, and this activity showed many of the expected properties of a picornavirus 3Dpol. A number of HAV-specific polypeptides were detected in these cells, and it is unclear which of these was responsible for the polymerase activity.