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Cell cycle control of polyomavirus-induced transformation
1Department of Microbiology, Michigan State University, East Lansing 48824-1101.
Journal of Virology
|April 1, 1993
Summary
Polyomavirus transformation efficiency in Fischer rat cells significantly depends on the cell cycle stage. Early G1 phase infections show higher transformation rates than G2, regulated by viral adsorption and early gene expression steps.
Area of Science:
- Virology
- Cell Biology
- Cancer Research
Background:
- Polyomavirus transformation is a key area in cancer research.
- Understanding viral infection mechanisms in relation to the host cell cycle is crucial.
Purpose of the Study:
- To investigate the cell cycle dependence of polyomavirus transformation.
- To identify specific stages of the viral life cycle regulated by the cell cycle.
Main Methods:
- Infection of nonpermissive Fischer rat cells (FR3T3) released from G0.
- Analysis of transformation frequencies at different cell cycle phases (G1 vs. G2).
- Quantification of early viral gene expression and viral adsorption.
Main Results:
- A 5- to 100-fold difference in transformation frequency was observed between early G1 and G2 infected cells.
- Cell cycle regulation affects viral adsorption (2- to 15-fold) and early viral gene expression.
- Viral gene expression occurs as a delayed, synchronous burst in G1-infected cells.
Conclusions:
- Polyomavirus infection and transformation in FR3T3 cells are regulated by the cell cycle at multiple early steps.
- These include viral adsorption and events preceding or including early gene transcription.
- A model involving c-fos and c-jun is proposed to explain these cell cycle-dependent mechanisms.