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A new method for rapidly removing contaminating micro-organisms from porcine parvovirus or pseudorabies virus
E Rivera1, K O Grönvik, K A Karlsson
1National Veterinary Institute, Division of Vaccine Research, Uppsala, Sweden.
Vaccine
|January 1, 1993
Abstract:
Virus-contaminated cell cultures are a major problem in the bio-industry. Methods employed to date to remove contaminating micro-organisms are slow and costly, and a new method is proposed here which is simple and rapid. The method uses polyacrylamide beads coated with specific antibodies which yielded bead-antibody-virus complexes when suspended in the virus solution to be cleared. The purified virus was propagated in cells which show phagocytic activity. Vaccine master-seed virus is shown to be rapidly cleaned and propagated using this method.