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Exogenous cGMP prevents decrease in diuresis and natriuresis induced by inhibition of NO synthesis

V Lahera1, J Navarro, M L Biondi

  • 1Department of Physiology, Faculty of Medicine, Universidad Complutense, Madrid, Spain.

The American Journal of Physiology
|February 1, 1993
PubMed
Summary

The study shows that 8-bromo-cGMP (8-BrcGMP) can prevent the negative effects of L-NAME on blood pressure and kidney function in rats. Higher doses of 8-BrcGMP fully protected against L-NAME-induced changes.

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Area of Science:

  • Nephrology
  • Cardiovascular Physiology
  • Pharmacology

Background:

  • Nitric oxide (NO) synthesis inhibition by L-NAME increases blood pressure and impairs renal function.
  • Cyclic guanosine monophosphate (cGMP) plays a role in regulating cardiovascular and renal hemodynamics.

Purpose of the Study:

  • To investigate the protective effects of 8-bromo-cGMP (8-BrcGMP), a cGMP analogue, against L-NAME-induced alterations in mean arterial pressure (MAP) and renal function in rats.

Main Methods:

  • Intravenous infusion of L-NAME to induce changes in MAP and renal parameters (RPF, GFR, UV, UNaV).
  • Co-administration of varying doses of 8-BrcGMP with L-NAME.
  • Administration of dibutyryl cAMP with L-NAME to assess specificity.

Main Results:

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  • L-NAME infusion significantly increased MAP and reduced RPF, GFR, UV, and UNaV.
  • Co-infusion of 8-BrcGMP (100 µg/kg/min) with L-NAME prevented changes in MAP, RPF, and GFR but not UV and UNaV.
  • Co-infusion of a higher dose of 8-BrcGMP (200 µg/kg/min) with L-NAME prevented all observed changes in MAP and renal function.
  • Dibutyryl cAMP did not prevent L-NAME-induced alterations, indicating the specificity of 8-BrcGMP's effects.

Conclusions:

  • 8-bromo-cGMP effectively prevents L-NAME-induced increases in blood pressure and reductions in renal hemodynamics and excretory function in rats.
  • The protective effects are dose-dependent and specific to cGMP signaling pathways.