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Topoisomerase II function detected as protein-DNA complexes in two sublines of HL-60 cells
1Medizinische Poliklinik, Universität Würzburg, Germany.
Toxicology Letters
|April 1, 1993
Abstract:
Topoisomerase II forms a cleavable complex with DNA as an intermediate in the topoisomerization reaction. Denaturation produces covalent linkage between the enzyme and DNA, and produces double-stranded DNA breaks at the site of attachment. Thereby topoisomerase function can be detected by measuring the protein-DNA complexes. A whole cell filter binding assay exploiting this mechanism is described. m-AMSA-resistant HL-60 cells have higher protein-DNA complex formation than m-AMSA-sensitive HL-60 cells, suggesting different activities of topoisomerase II in drug-resistant vs. drug-sensitive cells.