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Updated: Aug 12, 2026

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
Published on: June 26, 2013
Coactivation of metabotropic and NMDA receptors is required for LTP induction
M A Musgrave1, B A Ballyk, J W Goh
1Department of Pharmacology and Toxicology, Queen's University, Kingston, Ontario, Canada.
Abstract:
Induction of long-term potentiation (LTP) of synaptic transmission in the CA1 region of the hippocampus is dependent on N-methyl-D-aspartate (NMDA) receptor activation. However, NMDA receptor stimulation alone is not adequate to trigger LTP. Results from the present study indicate that, in addition to NMDA receptors, there is a requirement for co-stimulation of a postsynaptic metabotropic glutamate receptor (mGluR). The mGluR involved in eliciting LTP is not antagonized by L-2-amino-3-phosphonopropionic acid (L-AP3, 500 microM), nor is it coupled to phosphoinositide metabolism. We demonstrate that a novel mGluR, which is stimulated by trans-ACPD and is linked to increase in adenosine 3',5'-monophosphate (cAMP), is physiologically relevant to the induction of LTP.
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