Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superoxide as an intracellular radical sink

C C Winterbourn1

  • 1Department of Pathology, Christchurch School of Medicine, New Zealand.

Free Radical Biology & Medicine
|January 1, 1993
PubMed
Summary

This study proposes a radical pathway where superoxide generates oxidative stress by converting reduced glutathione (GSH) to oxidized glutathione (GSSG). Superoxide dismutase is crucial for preventing this stress and protecting cells from radical damage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Assay of superoxide dismutase activity in a plate assay using WST-1" [Free Radic. Biol. Med. 103 (2017) 188-191].

Free radical biology & medicine·2017
Same author

Myeloperoxidase: a key regulator of neutrophil oxidant production.

Redox report : communications in free radical research·2016
Same author

A monoclonal antibody recognizing the chlorohydrin derivatives of oleic acid for probing hypochlorous acid involvement in tissue injury.

Redox report : communications in free radical research·2016
Same author

High plasma thiocyanate levels are associated with enhanced myeloperoxidase-induced thiol oxidation and long-term survival in subjects following a first myocardial infarction.

Free radical research·2014
Same author

Association of hydrogen peroxide in exhaled breath condensates from infants with respiratory distress syndrome with the development of chronic lung disease.

Archives of disease in childhood. Fetal and neonatal edition·2006
Same author

Vitamin C supplementation in very preterm infants: a randomised controlled trial.

Archives of disease in childhood. Fetal and neonatal edition·2005

Area of Science:

  • Biochemistry
  • Cellular Biology
  • Oxidative Stress Research

Background:

  • Intracellularly generated radicals pose a threat to cellular integrity.
  • Reduced glutathione (GSH) plays a key role in cellular defense mechanisms.
  • Superoxide is a reactive oxygen species with significant biological implications.

Purpose of the Study:

  • To propose a novel metabolic pathway involving superoxide and GSH.
  • To elucidate the role of superoxide in generating oxidative stress.
  • To highlight the protective function of superoxide dismutase (SOD) in this context.

Main Methods:

  • Theoretical pathway proposal.
  • Analysis of radical transfer mechanisms.
  • Examination of redox cycling reactions involving GSH and oxygen.

Main Results:

  • Superoxide can act as a sink for radicals, initiating chain reactions with GSH.
  • These reactions lead to excessive production of oxidized glutathione (GSSG) and hydrogen peroxide.
  • This process results in significant oxidative stress, depleting cellular reducing equivalents and energy.

Conclusions:

  • Superoxide dismutase is essential for mitigating superoxide-induced oxidative stress and direct radical damage.
  • GSH and superoxide dismutase are functionally linked in antioxidant defense.
  • The proposed pathway explains how superoxide and SOD influence cellular redox state and regulate redox-controlled functions.

Related Experiment Videos