Emerging relationships between cytochemical properties and sensory modality transmission in primary sensory neurons

Primary sensory neurons have been categorized according to a variety of characteristics, including modality responsiveness, somal size, cytology, cytochemistry, and the organization of their central axon collateral arborizations. A major aim in the study of primary afferents has been to determine the relationships between dorsal root ganglia neuronal physiology, anatomy, and chemistry that could provide a basis for a classification scheme more directly relevant to function. Here we briefly review these relationships and examine the utility of specific histochemical and immunohistochemical markers representative of distinct populations of neurons that may transmit particular sensory modalities. In addition, we discuss some of our observations suggesting that one population of dorsal root ganglia neurons contains high levels of cytochrome oxidase, carbonic anhydrase, parvalbumin, and calbindin D28k, while a separate population contains fluoride-resistant acid phosphatase, calcitonin gene-related peptide, and displays immunoreactivity with an antibody that labels the central arborization of a specific class of unmyelinated afferents in the dorsal horn. This may have implications for the combinations of substances contained within neurons with distinct sensory functions.