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The mariner transposable element is widespread in insects

H M Robertson1

  • 1Department of Entomology, University of Illinois, Urbana 61801.

Nature
|March 18, 1993
PubMed
Summary

The mariner transposable element, found across diverse insect species, shows potential for genetic transformation systems. This DNA-based element exhibits significant diversity, suggesting both inheritance and horizontal transfer.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • The mariner transposable element is a DNA-based mobile genetic element belonging to the short inverted terminal repeat class.
  • It was initially identified in Drosophila mauritiana and has since been found in other Drosophilidae species and the moth Hyalophora cecropia.

Purpose of the Study:

  • To investigate the presence and diversity of mariner elements in a broader range of insect species.
  • To assess the potential of mariner elements for developing genetic transformation systems in non-drosophilid insects.

Main Methods:

  • Design of primers based on conserved amino acid regions of putative mariner transposase genes from D. mauritiana and H. cecropia.
  • Amplification of mariner element sequences from ten insect species across six orders, including Anopheles gambiae.
  • Sequence analysis of multiple clones from each species to identify mariner element diversity and potential functional elements.

Main Results:

  • Discovery of diverse mariner elements in multiple insect orders, with evidence of multiple subfamilies within some insect genomes.
  • Identification of intact open reading frames in mariner element clones from each species, suggesting the presence of functional transposable elements.
  • Demonstration of both vertical inheritance and horizontal transfer as mechanisms for mariner element distribution.

Conclusions:

  • The mariner transposable element is widespread across diverse arthropod taxa.
  • The presence of intact open reading frames indicates potential functionality.
  • Mariner elements are promising candidates for developing genetic transformation technologies in various insect species and potentially other arthropods.

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