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Enhanced immunosuppressive activity associated with metastatic lymphoma cells
W Hao1, T L McDonald, K W Brunson
1Department of Cell Biology, University of Nebraska Medical Center, Omaha 68198-6395.
Cancer Research
|April 15, 1993
Summary
Highly metastatic lymphoma cells (RAW117-H10) release immunosuppressive molecules that inhibit immune cell proliferation and function. These molecules may aid tumor growth and metastasis in vivo.
Area of Science:
- Immunology
- Cancer Biology
- Molecular Biology
Background:
- Previous studies demonstrated that highly metastatic RAW117-H10 cells, unlike less metastatic RAW117-P cells, suppress T-cell and B-cell proliferation.
- This immunosuppression was also observed using butanol-extracted cell surface molecules from RAW117-H10 cells.
Purpose of the Study:
- To characterize the immunosuppressive properties of RAW117-H10 cell surface molecules on various immune functions.
- To isolate and purify the specific molecule(s) responsible for the observed immunosuppression.
Main Methods:
- Butanol extraction of RAW117-H10 cell surface molecules.
- Assays for natural killer (NK) cell and lymphokine-activated killer (LAK) cell cytotoxicity.
- Bone marrow colony formation assays (granulocyte-macrophage and fibroblast).
- Interleukin-2 (IL-2) production assays.
- Preparative isoelectric focusing for molecule purification.
Main Results:
- The immunosuppressive molecules from RAW117-H10 cells inhibited NK cell and LAK cell cytotoxicity.
- These molecules suppressed granulocyte-macrophage colony formation but not fibroblast colony formation.
- Interleukin-2 production by T-lymphocytes was inhibited.
- A suppressive molecule with an isoelectric point of 4.3 and molecular weight of approximately 70,000 was isolated.
Conclusions:
- Metastatic RAW117-H10 lymphoma cells express and release immunosuppressive molecules.
- These molecules likely contribute to the tumor's ability to evade immune responses and facilitate metastasis in vivo.