Rapid preparation of subsarcolemmal and interfibrillar mitochondrial subpopulations from cardiac muscle
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J M Chemnitius1, T Manglitz, M Kloeppel
1Medizinische Klinik, Georg-August-Universität, Göttingen, Germany.
The International Journal of Biochemistry
|April 1, 1993
Abstract:
1. Subsarcolemmal and interfibrillar mitochondria were prepared with complete recovery from rabbit and porcine heart muscle by upward-flotation during 60 sec of Percoll density gradient centrifugation. 2. Mitochondrial subpopulations were identified and characterized according to buoyant density, electron-microscopy, marker enzyme activities and respiratory performance. 3. ADP-induced state 3-respiration related to latent citrate synthase activity as a marker for structurally intact mitochondria was not significantly different in both mitochondrial subtypes.