Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-resolved optical spectroscopy on intact myocytes

G Antonini1, F Malatesta, P Sarti

  • 1Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata.

Cardioscience
|March 1, 1993
PubMed
Summary

Researchers studied rat heart cells using spectroscopy to understand electron transfer in cellular respiration. They found that internal electron transfer from cytochrome a to cytochrome a3 limits cytochrome oxidase activity in living cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous Enchondroma and Osteochondroma in the Hand: A Case Report.

La Clinica terapeutica·2025
Same author

The role of the urinary microbiome on male benign prostatic hyperplasia (BPH) and its management using probiotic supplementation: a narrative review.

European review for medical and pharmacological sciences·2024
Same author

Helical PHILOS plating in shaft humeral fractures with proximal extension: a safe choice using an anterior approach.

La Clinica terapeutica·2024
Same author

A two-window mini-open approach for suprapectoral tenodesis for traumatic ruptures of the long head of the biceps tendon.

La Clinica terapeutica·2023
Same author

Distal Tibia Fractures: is the Tibia First Technique a Rational Approach?

Malaysian orthopaedic journal·2023
Same author

Diagnosis and management of a superior gluteal artery lesion following intramedullary fixation of a diaphyseal femoral fracture: a case report.

La Clinica terapeutica·2022

Area of Science:

  • Cellular respiration
  • Mitochondrial function
  • Spectroscopic analysis

Background:

  • Cardiac myocytes are fundamental units of heart function.
  • Understanding mitochondrial electron transport is crucial for cellular energy production.
  • Cytochrome oxidase plays a key role in the electron transport chain.

Purpose of the Study:

  • To investigate the redox state of cytochromes c and a in living cardiac myocytes.
  • To determine the rate-limiting step in cytochrome oxidase turnover within intact cells.
  • To apply spectroscopic methods for analyzing chromophore behavior in cellular environments.

Main Methods:

  • Spectroscopic studies on isolated rat heart myocytes using a stopped-flow apparatus.
  • Employing exogenous reductants (sodium ascorbate, tetramethyl-para-phenylenediamine) for membrane permeability assessment.

Related Experiment Videos

  • Utilizing singular value decomposition for spectral resolution.
  • Manipulating carbon monoxide levels to optimize spectral analysis of myoglobin and cytochrome c oxidase.

    • Main Results:

    • Isolated myocytes remained viable for the duration of the experiments.
    • Tetramethyl-para-phenylenediamine permeated both cytoplasmic and mitochondrial membranes.
    • Singular value decomposition effectively resolved spectral contributions of chromophores.
    • Cytochrome c and cytochrome a exhibited similar redox states (25-30% oxidized) during steady-state respiration and simultaneous changes during oxygen exhaustion.
    • Internal electron transfer from cytochrome a to cytochrome a3 was identified as the rate-limiting step.

    Conclusions:

    • The study provides insights into the functional dynamics of cytochrome oxidase in living cardiac cells.
    • Electron transfer between cytochrome a and cytochrome a3 is the bottleneck for cytochrome oxidase activity.
    • Spectroscopic techniques, enhanced by SVD and CO manipulation, are effective for studying mitochondrial components in situ.