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Updated: Aug 17, 2026
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
Isolation and characterization of a new nucleoside antibiotic, amipurimycin
Abstract:
A new antibiotic amipurimycin, active against Pyricularia oryzae in vitro and in vivo, was isolated from the culture filtrate of Streptomyces novoguineensis nov. sp. The antibiotic was purified by a combination of ion-exchange and adsorption chromatography based on its amphoteric water-soluble characteristics. Its molecular formula was estimated to be C20H27approximately31N7O8-H2O. Characteristic maxima in the UV spectrum and signals in the PMR and CMR spectra were similar to those of 2-aminopurine 9-(beta-D)-riboside. These findings indicated that amipurimycin is a new nucleoside antibiotic and the first example of a natural product containing 2-aminopurine.
Insights
A novel antibiotic, amipurimycin, was discovered from Streptomyces novoguineensis. This new nucleoside antibiotic shows activity against Pyricularia oryzae, offering potential in agricultural applications.
Area of Science:
- Microbiology
- Natural Product Chemistry
- Biochemistry
Background:
- Pyricularia oryzae causes significant crop diseases.
- The search for novel antimicrobial agents is crucial for disease management.
- Streptomyces species are a rich source of bioactive compounds.
Purpose of the Study:
- To isolate and characterize a new antibiotic from Streptomyces novoguineensis.
- To evaluate the efficacy of the new antibiotic against Pyricularia oryzae.
- To determine the chemical nature of the isolated compound.
Main Methods:
- Isolation of the antibiotic from culture filtrate.
- Purification using ion-exchange and adsorption chromatography.
- Structural elucidation using UV, PMR, and CMR spectroscopy.
Main Results:
- Amipurimycin was isolated and purified.
- The antibiotic demonstrated in vitro and in vivo activity against Pyricularia oryzae.
- Its molecular formula was estimated as C20H27approximately31N7O8·H2O.
- Spectroscopic data suggested amipurimycin is a nucleoside containing 2-aminopurine.
Conclusions:
- Amipurimycin is a novel nucleoside antibiotic.
- It is the first natural product identified containing 2-aminopurine.
- Amipurimycin shows promise for controlling Pyricularia oryzae infections.
