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Related Experiment Videos

Bone-derived growth factor release from poly(alpha-hydroxy acid) implants in vitro

M C Meikle1, W Y Mak, S Papaioannou

  • 1Department of Orthodontics, University of London, UK.

Biomaterials
|February 1, 1993
PubMed
Summary

Bone matrix extracts incorporated into poly(DL-lactide-co-glycolide) implants release growth factors and promote porosity for potential bone regeneration. These implants show promise for stimulating osseous regeneration in vivo.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Orthopedic Research

Background:

  • Poly(alpha-hydroxy acid) polymers are widely used in biomedical applications.
  • Bone matrix extracts contain crucial growth factors for bone regeneration.
  • Developing effective delivery systems for bone growth factors is essential for osseous regeneration.

Purpose of the Study:

  • To evaluate the release of biological activity from bone matrix extracts (BMX) incorporated into poly(DL-lactide-co-glycolide) (PLGA) implants.
  • To assess the structural changes, specifically porosity, of these implants over time.
  • To determine the potential of these composite implants for stimulating bone ingrowth and osseous regeneration.

Main Methods:

  • Bovine cortical bone matrix proteins were extracted and incorporated into PLGA polymer discs.

Related Experiment Videos

  • BMX-loaded PLGA implants were cultured in vitro for 5 weeks.
  • Growth factor release was quantified by measuring fibroblast proliferation.
  • Scanning electron microscopy (SEM) was used to analyze implant porosity.

    • Main Results:

    • A significant portion (60-75%) of biological activity was released within the first week of culture.
    • Less than 50% of the incorporated growth factor units retained biological activity post-release.
    • SEM revealed significant internal porosity development by week 2, with channels suitable for bone ingrowth.
    • The polymer implants demonstrated structural changes conducive to bone regeneration.

    Conclusions:

    • PLGA implants effectively incorporate and release bone-derived growth factors.
    • The developed implants exhibit porosity suitable for facilitating bone ingrowth.
    • These findings suggest that poly(alpha-hydroxy acid) polymers with bone growth factors hold potential for in vivo osseous regeneration.