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Design of primers for PCR amplification of highly variable genomes
J Dopazo1, A Rodríguez, J C Sáiz
1INIA-Sanidad Animal, Madrid, Spain.
Computer Applications in the Biosciences : CABIOS
|April 1, 1993
Abstract:
A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.