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Related Experiment Videos

Anti-histone autoantibodies react specifically with the B cell surface

S Mécheri1, G Dannecker, D Dennig

  • 1Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.

Molecular Immunology
|April 1, 1993
PubMed
Summary

Researchers identified a monoclonal antibody, 5B9.6, that specifically targets histones on the surface of B cells. This antibody also reacts with human B cells, suggesting potential applications in immunology and diagnostics.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The study aimed to generate antibodies against Mls-1a antigens.
  • Monoclonal antibody 5B9.6 was selected for detailed investigation.

Purpose of the Study:

  • To characterize the specificity of the 5B9.6 monoclonal antibody.
  • To investigate the potential of 5B9.6 as a biomarker for B cells.

Main Methods:

  • Flow cytometry (FACS) analysis
  • Enzyme immunoassays
  • Immunoprecipitation
  • Peptide sequencing
  • Cell culture with IL-1 and cAMP stimulation

Main Results:

  • 5B9.6 antibody specifically reacts with B cells across different mouse strains.

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  • The antibody targets histones, with 100% homology to H2B histones found in immunoprecipitated polypeptides.
  • 5B9.6 interacts with the 7OZ/3 pre-B cell line, and histone presence is modulated by IL-1 and cAMP.
  • The antibody also binds to human peripheral blood B cells.

    • Conclusions:

    • The 5B9.6 monoclonal antibody is a specific reagent for detecting histones on B cells.
    • This antibody shows cross-reactivity with human B cells, indicating potential diagnostic and research applications.