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Related Experiment Videos

Changes in serotonin-induced potentials during spinal cord development

L Ziskind-Conhaim1, B S Seebach, B X Gao

  • 1Department of Physiology, University of Wisconsin, Madison 53706.

Journal of Neurophysiology
|April 1, 1993
PubMed
Summary

Serotonin (5-hydroxytryptamine, 5-HT) excites spinal neurons early in development. Motoneuron responses to 5-HT mature as 5-HT projections grow into the ventral horn, mediated by multiple receptor subtypes.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Neuropharmacology

Background:

  • Serotonin (5-hydroxytryptamine, 5-HT) plays a crucial role in spinal cord development and function.
  • Understanding the developmental timeline of 5-HT actions on motoneurons is essential for comprehending motor control development.

Purpose of the Study:

  • To investigate the developmental changes in motoneuron responses to serotonin (5-HT).
  • To characterize the growth pattern of 5-HT projections into the ventral horn of the embryonic and neonatal rat spinal cord.
  • To elucidate the receptor subtypes involved in mediating 5-HT's effects on spinal neurons during development.

Main Methods:

  • Intracellular recordings from lumbar motoneurons in isolated rat spinal cords at different embryonic and postnatal ages.

Related Experiment Videos

  • Pharmacological manipulations using 5-HT agonists and antagonists, tetrodotoxin, and high Mg2+.
  • Analysis of 5-HT projection growth patterns using anatomical localization.

    • Main Results:

    • 5-HT projections appeared in the lumbar spinal cord by E16-E17 and reached motoneuron somata by postnatal day 1-2.
    • Early motoneuron responses to 5-HT (E16-E17) were slow-rising depolarizations; post-E18, responses became long-lasting depolarizations with increased membrane resistance.
    • Postnatally, 5-HT induced significant depolarization and repetitive firing, mediated by direct action on motoneurons and involving multiple 5-HT receptor subtypes.
    • 5-HT consistently reduced dorsal root-evoked potentials across all developmental stages.

    Conclusions:

    • 5-HT excites spinal neurons even before its projections innervate the ventral horn.
    • The nature of 5-HT-induced motoneuron responses evolves significantly with the maturation of 5-HT innervation.
    • Multiple 5-HT receptor subtypes mediate the diverse actions of 5-HT on developing spinal neurons.