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Updated: Aug 15, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
The MAP kinase cascade is essential for diverse signal transduction pathways
Abstract:
Mitogen-activated protein (MAP) kinases are activated by combined tyrosine and threonine phosphorylation catalysed by MAP kinase kinase, a novel class of protein kinases with dual specificity for both tyrosine and serine/threonine. MAP kinase kinase is turned on by serine/threonine phosphorylation catalysed by an immediate upstream kinase. The MAP kinase cascade appears to be conserved during evolution and thus might play an essential role in diverse intracellular signaling processes from yeasts to vertebrates.
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