Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Restriction landmark genomic scanning method and its various applications

Y Hayashizaki1, S Hirotsune, Y Okazaki

  • 1Department of Bioscience, National Cardiovascular Center Research Institute, Osaka, Japan.

Electrophoresis
|April 1, 1993
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gene expression of angiotensin II receptor in blood cells of Cushing's syndrome.

Hypertension (Dallas, Tex. : 1979)·1995
Same author

Attenuation of glycogenolytic action of activin A in intact rat liver.

The American journal of physiology·1995
Same author

[Ictal electroencephalographic recordings of patients with seizure].

Rinsho byori. The Japanese journal of clinical pathology·1995
Same author

Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells.

The Journal of biological chemistry·1995
Same author

[Clinicopathological studies of anti-HCV P1P4 core antibody].

Rinsho byori. The Japanese journal of clinical pathology·1995
Same author

Seroprevalence of hepatitis B and C viruses in eastern Nepal.

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases·1994

We developed restriction landmark genomic scanning (RLGS), a new genome scanning method. RLGS efficiently analyzes DNA copy number and methylation status across various organisms and biological fields.

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Understanding genome structure and function is crucial.
  • Existing methods for scanning genomic DNA states have limitations.
  • Need for high-throughput, versatile genomic analysis techniques.

Purpose of the Study:

  • Introduce a novel genome scanning method: Restriction Landmark Genomic Scanning (RLGS).
  • Detail the principles and advantages of RLGS.
  • Demonstrate the broad applicability of RLGS in biological research.

Main Methods:

  • Developed RLGS based on restriction enzyme sites as landmarks.
  • Utilized direct end labeling of genomic DNA.
  • Employed high-resolution two-dimensional electrophoresis.

Related Experiment Videos

  • Introduced RLGS-M for analyzing DNA methylation using methylation-sensitive enzymes.

    • Main Results:

    • RLGS enables high-speed scanning of thousands of restriction landmarks.
    • Method allows for extension of scanning field and application to any organism.
    • Spot intensity reflects DNA copy number, distinguishing haploid and diploid states.
    • RLGS can detect genomic alterations like amplification and deletion.
    • RLGS-M effectively screens for methylated DNA sites.

    Conclusions:

    • RLGS is a versatile and efficient method for analyzing physical genomic DNA states.
    • The technique is applicable across diverse biological fields, including cancer research and developmental biology.
    • RLGS provides valuable insights into DNA copy number variations and methylation patterns.