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Related Experiment Videos

Physical and functional interaction between WT1 and p53 proteins

S Maheswaran1, S Park, A Bernard

  • 1Massachusetts General Hospital Cancer Center, Charlestown.

Proceedings of the National Academy of Sciences of the United States of America
|June 1, 1993
PubMed
Summary
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The Wilms tumor suppressor WT1 protein physically interacts with p53. This interaction alters their functions, with WT1 activating EGR1 without p53, and cooperating with p53 on other targets.

Area of Science:

  • Molecular Biology
  • Cancer Genetics
  • Developmental Biology

Background:

  • Wilms tumor (WT) is a pediatric kidney cancer linked to the WT1 tumor suppressor gene.
  • WT1 encodes a transcription factor regulating gene expression.
  • p53 is a critical tumor suppressor with widespread expression.

Purpose of the Study:

  • To investigate the physical interaction between WT1 and p53.
  • To determine how this interaction affects their transcriptional activities.

Main Methods:

  • Transfection of cells to express WT1 and p53.
  • Co-immunoprecipitation to assess physical association.
  • Reporter gene assays to measure transcriptional activity.

Main Results:

Related Experiment Videos

  • WT1 and p53 physically associate in transfected cells.
  • In the absence of p53, WT1 activates transcription of the EGR1 site.
  • WT1 enhances p53's transactivation of the muscle creatine kinase promoter.

Conclusions:

  • WT1 and p53 interaction modulates their distinct transcriptional functions.
  • WT1's role shifts from repressor to activator depending on p53 presence.
  • This interplay is crucial for understanding WT1's tumor-suppressive mechanisms.