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Updated: Aug 14, 2026
10:59
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Published on: August 21, 2021
Urea facilitates quantitative estimation of DNA damage in situ by preventing its degradation
1Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Analytical Biochemistry
|May 1, 1993
Abstract:
A buffer containing 7 M urea was used to successfully prevent DNA degradation of in situ damaged fish skin and resulted in reproducible high molecular weight DNA. This treatment in combination with a quantitative gel electrophoresis technique permitted estimation of small changes induced in unlabeled DNA per unit molecular weight after treatment with a DNA damaging agent (e.g., ultraviolet irradiation).
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