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Related Experiment Videos

Urinary thrombomodulin, its isolation and characterization

S Yamamoto1, T Mizoguchi, T Tamaki

  • 1Tokyo Research Laboratories, Kowa Co., Ltd.

Journal of Biochemistry
|April 1, 1993
PubMed
Summary
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Protein C activation by recombinant thrombomodulin in plasma.

Biological & pharmaceutical bulletin·1998

Two urine thrombomodulin fragments (types I and II) were isolated and characterized. Both fragments retain thrombin inhibition and protein C activation functions, with distinct activities.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Urology

Background:

  • Thrombomodulin is a crucial protein involved in regulating coagulation.
  • Thrombomodulin fragments are found in urine, but their properties are not fully understood.

Purpose of the Study:

  • To isolate and characterize major molecular forms of thrombomodulin fragments in human urine.
  • To compare the functional activities of these urinary thrombomodulin fragments.

Main Methods:

  • Isolation of thrombomodulin fragments using four sequential column chromatography steps.
  • Characterization using SDS-PAGE, isoelectric focusing, and amino acid sequencing.
  • Assessment of thrombin inhibition and protein C activation activities.

Main Results:

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  • Two major forms, type I (98 kDa) and type II (90 kDa), were identified.
  • Both forms share an identical 468 amino acid sequence, lacking the carboxyl-terminal region.
  • Type II fragments exhibit lower galactosamine content than type I.
  • Both types showed reduced but significant thrombin inhibition and protein C activation compared to intact thrombomodulin.
  • Type I demonstrated stronger thrombin clotting inhibition, while type II was a more effective cofactor for protein C activation.

Conclusions:

  • Human urine contains distinct thrombomodulin fragments with preserved, albeit reduced, anticoagulant functions.
  • Structural differences, particularly in glycosylation, influence the functional activities of these fragments.
  • Urinary thrombomodulin fragments may play a role in local hemostasis or serve as biomarkers.