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Molecular cloning and expression of a rat cDNA encoding MDCK-type chloride channel
1Department of Clinical Biology and Hormonal Regulation, Tohoku University School of Medicine, Sendai, Japan.
Biochimica Et Biophysica Acta
|June 25, 1993
Abstract:
A cDNA encoding MDCK-type chloride channel was cloned from the rat kidney cDNA library using the homology screening approach. The deduced amino acid sequence of cloned cDNA has a 92.4% homology with the MDCK chloride channel, and electrophysiological analysis using Xenopus oocyte expression system showed the functional character of chloride channel. The rat kidney chloride channel, abbreviated RKCL, is not specific to the kidney, but widely expressed in various tissues. It is suggested that RKCL is important for most cells.