Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase radioreceptor assay for paralytic shellfish toxins

M R Vieytes1, A G Cabado, A Alfonso

  • 1Departmento de Fisiologiá, Facultad de Veterinaria, Lugo, Spain.

Analytical Biochemistry
|May 15, 1993
PubMed
Summary

A new assay uses rat brain sodium channels to detect paralytic shellfish poisoning (PSP) toxins. This method offers a sensitive way to identify toxin-contaminated mussels, crucial for public health and food safety.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure to the COVID-19 pandemic environment and generosity.

Royal Society open science·2022
Same author

It's not just an ileus: disparities associated with ileus following ventral hernia repair.

Hernia : the journal of hernias and abdominal wall surgery·2020
Same author

Improvement of appropriate pharmacological prophylaxis in hospitalised cancer patients with a multiscreen e-alert system: a single-centre experience.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2018
Same author

The impact of depuration on mussel hepatopancreas bacteriome composition and predicted metagenome.

Antonie van Leeuwenhoek·2018
Same author

A clinical trial for patients with acute myeloid leukemia or myelodysplastic syndromes not eligible for standard clinical trials.

Leukemia·2017
Same author

A clinical trial for patients with acute myeloid leukemia or myelodysplastic syndromes not eligible for standard clinical trials.

Leukemia·2016

Area of Science:

  • Neuroscience
  • Toxicology
  • Biochemistry

Background:

  • Paralytic Shellfish Poisoning (PSP) poses a significant public health risk.
  • Accurate and sensitive detection of PSP toxins is essential for food safety.
  • Existing detection methods may have limitations in speed or sensitivity.

Purpose of the Study:

  • To develop a direct solid-phase binding assay for detecting PSP toxins.
  • To utilize sodium channels from rat brain membranes for toxin detection.
  • To evaluate the assay's sensitivity and applicability for identifying contaminated mussels.

Main Methods:

  • Coating rat brain membrane preparations onto microtiter plates.
  • Using tritiated saxitoxin ([3H]-saxitoxin; STX) as a labeled sodium channel blocker.

Related Experiment Videos

  • Measuring competitive displacement of [3H]STX by other PSP toxins to quantify toxin levels.

    • Main Results:

    • The assay demonstrated effective binding of PSP toxins to rat brain membranes.
    • The concentration of STX and tetrodotoxin required to displace 50% of bound [3H]STX was determined (1.7 and 1.76 ng/ml, respectively).
    • The IC50 values for PSP toxins in contaminated mussel samples ranged from 0.03 to 0.30 ng/ml, indicating high sensitivity.

    Conclusions:

    • The developed direct solid-phase binding assay is a potentially useful method for detecting PSP toxins.
    • This assay shows promise for identifying mussels contaminated with toxins from red tides.
    • The method provides a sensitive approach for monitoring shellfish safety.