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Production of recombinant Xenopus fibroblast growth factor receptor-1 using a baculovirus expression system
1Dept. of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, MD 20855.
Biochemical and Biophysical Research Communications
|June 30, 1993
Abstract:
A baculovirus expression system has been used to express large quantities of full-length, biologically active Xenopus fibroblast growth factor receptor-1. Recombinant XFGFR-1 was purified to near homogeneity by a single step immunoaffinity purification procedure. The recombinant XFGFR-1 binds both FGF-1 and FGF-2 with high affinity. XFGFR-1 undergoes autophosphorylation principly on tyrosine residues in an immune-complex kinase assay. Thus, recombinant XFGFR-1 expressed in insect cells is biologically active and suitable for structural and functional analysis.