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Related Experiment Videos

pCMPS-induced changes in lens membrane permeability and transparency

J Sanderson1, G Duncan

  • 1School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.

Investigative Ophthalmology & Visual Science
|July 1, 1993
PubMed
Summary

Externally facing sulfhydryl groups in lens membranes control permeability. Targeting these with agents like pCMPS can induce age-related cation changes and opacification, suggesting new anticataract strategies.

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Area of Science:

  • Ocular Biophysics
  • Membrane Physiology
  • Cataract Research

Background:

  • Lens transparency and permeability are crucial for vision.
  • Externally facing membrane protein sulfhydryl groups are implicated in lens function.
  • Age-related changes in the lens can lead to opacity and vision impairment.

Purpose of the Study:

  • To investigate the role of lens membrane sulfhydryl groups in controlling permeability and transparency.
  • To examine the effects of p-chloromercuriphenyl sulfonate (pCMPS) on lens electrical properties and ion fluxes.
  • To quantify pCMPS-induced opacification.

Main Methods:

  • Measuring lens voltage and resistance to assess membrane permeability changes.
  • Monitoring 22Na+ and 45Ca2+ fluxes to track ion movements.

Related Experiment Videos

  • Using ion-selective microelectrodes to measure internal free calcium.
  • Quantifying lens opacification via analysis of back-scattered light.

    • Main Results:

    • pCMPS (above 1 microM) depolarized membrane potential and decreased resistance.
    • Significant increases in 22Na+ and 45Ca2+ influxes were observed with pCMPS treatment.
    • Lens transparency decreased, particularly in the bow region.
    • Quinine mitigated pCMPS-induced ion flux and opacification.

    Conclusions:

    • pCMPS at low concentrations mimics age-related cation permeability changes in the lens.
    • Quinine's ability to ameliorate pCMPS effects suggests potential anticataract strategies targeting membrane proteins.
    • This research opens novel avenues for developing membrane-based interventions for cataracts.