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Related Experiment Videos

Actin depolymerisation induces process formation on MAP2-transfected non-neuronal cells

K Edson1, B Weisshaar, A Matus

  • 1Friedrich Miescher Institute, Basel, Switzerland.

Development (Cambridge, England)
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

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The microtubule-associated protein MAP2c stabilizes microtubules, promoting cell process outgrowth in nonneuronal cells. This effect involves interactions between microtubule bundles and the actin network, crucial for cell morphology.

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Molecular Neuroscience

Background:

  • Microtubules are essential cytoskeletal components involved in cell structure and transport.
  • Microtubule-associated proteins (MAPs) regulate microtubule stability, assembly, and organization.
  • The embryonic neuronal MAP2c has been shown to induce stable microtubule bundles in nonneuronal cells.

Purpose of the Study:

  • To investigate the role of MAP2c in inducing cell process outgrowth.
  • To elucidate the underlying mechanisms involving microtubule-actin interactions.
  • To understand how MAP2c influences microtubule properties like stability, bundling, and stiffness.

Main Methods:

  • Transfection of nonneuronal cells with MAP2c.
  • Treatment with cytochalasin B (actin depolymerizing drug) and taxol (microtubule stabilizing drug).

Related Experiment Videos

  • Video time-lapse microscopy to observe process outgrowth and retraction dynamics.

    • Main Results:

    • MAP2c transfection specifically induced microtubule-containing process outgrowth upon cytochalasin B treatment.
    • This effect was not observed in untransfected cells or cells treated with taxol.
    • Dynamic cycles of process outgrowth and retraction suggested interplay between microtubule bundles and cortical actin forces.

    Conclusions:

    • MAP2c confers stability, bundling, and stiffness to microtubules, promoting cell process outgrowth.
    • Stiffness likely results from MAP2c's unique tubulin-binding motifs limiting microtubule flexibility.
    • Similar MAPs (tau, MAP4) may similarly influence neuronal and glial cell morphology, contributing to neurite development.