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Published on: February 9, 2015
Promoter region of the rat phospholipase C-gamma 1 gene
1Department of Life Science, Pohang Institute of Science and Technology, Korea.
Abstract:
We have cloned the upstream region of the rat phospholipase C-gamma 1 gene and characterized its promoter activity. The 5'-upstream sequence is highly rich in GC, enriched with CpG dinucleotides and lacks a TATA motif. This sequence also contains many putative binding sites for regulatory proteins. Primer extension and RNase protection assay demonstrated a single transcriptional start site located at 103 nucleotides upstream of the ATG start codon. The transcriptional activities of various 5'-deleted fragments, fused with chloramphenicol acetyltransferase gene, were examined after transfection into C2C12 myoblast. Multiple positive and negative regulatory sites were observed.
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