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Related Experiment Videos

Autocrine motility factor is a growth factor

S Silletti1, A Raz

  • 1Metastasis Research Program, Michigan Cancer Foundation, Detroit, MI 48201.

Biochemical and Biophysical Research Communications
|July 15, 1993
PubMed
Summary

Autocrine motility factor (AMF), a cytokine secreted by cancer cells, acts as a growth factor. It stimulates fibroblast growth and motility, suggesting roles in tissue repair and cancer spread.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Autocrine motility factor (AMF) is a cytokine produced by cancer cells.
  • It regulates cell motility by binding to its receptor, gp78-AMFR, and activating a G-protein.
  • AMF's role as a growth factor and in paracrine signaling was previously unexplored.

Purpose of the Study:

  • To investigate the paracrine and mitogenic actions of Autocrine motility factor (AMF).
  • To explore the function of AMF as a growth factor in fibroblast models.
  • To understand the potential role of AMF in tissue regeneration and tumor cell dissemination.

Main Methods:

  • Purification of AMF from HT-1080 human fibrosarcoma cells.
  • Treatment of 3T3-A31-fibroblasts with purified AMF.
  • Assessment of cell growth, motility, and receptor (gp78-AMFR) expression.
  • Stimulation of experimentally wounded, density-arrested fibroblast monolayer cultures.

Main Results:

  • Purified AMF stimulated the growth and motility of 3T3-A31-fibroblasts at low concentrations (≤0.1 ng/ml).
  • Cell surface gp78-AMFR expression was down-regulated in contact-inhibited fibroblasts.
  • AMF promoted the healing of experimentally induced wounds in fibroblast cultures.

Conclusions:

  • AMF exhibits paracrine and mitogenic activities, functioning as a growth factor.
  • AMF's actions suggest a role in normal tissue regeneration processes.
  • The findings indicate a potential involvement of AMF in tumor cell dissemination.

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