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Spin trapping of superoxide released by opsonized asbestos from human promyelocytic leukemia cell line, HL60
T Takeuchi1, K Morimoto, H Kosaka
1Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, Japan.
Biochemical and Biophysical Research Communications
|July 15, 1993
Abstract:
By ESR using 5,5-dimethyl-1-pyrroline-1-oxide as a spin trap, superoxide (O2-) production was proved upon stimulation of dimethyl sulfoxide-differentiated HL60 by crocidolite opsonized with fresh or refrigerated serum, as well as by phorbol myristate acetate (PMA). Crocidolite, unopsonized or opsonized with frozen-thawed or heat-inactivated serum, did not induce O2- release. Addition of iron chelators or superoxide dismutase inhibited O2- release completely. Neither undifferentiated nor PMA-differentiated HL60 released O2- upon stimulation with opsonized crocidolite.