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Related Experiment Videos

Chorionic gonadotrophin secretion by human blastocysts

A Lopata1, K Oliva

  • 1Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia.

Human Reproduction (Oxford, England)
|June 1, 1993
PubMed
Summary

Supplements like human cord serum, insulin, transferrin, selenium (ITS), and platelet-derived growth factor (PDGF) can stimulate chorionic gonadotrophin (CG) secretion in early blastocysts, crucial for pregnancy. These findings suggest receptors for these factors are present on preimplantation embryos.

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Area of Science:

  • Reproductive biology
  • Embryology
  • Biochemistry

Background:

  • In-vitro fertilization (IVF) programs generate abnormal embryos.
  • Understanding early embryonic development and hormone production is critical for reproductive success.

Purpose of the Study:

  • To investigate the influence of various supplements on chorionic gonadotrophin (CG) secretion in cultured blastocysts.
  • To identify factors that can stimulate CG production in serum-free conditions.

Main Methods:

  • Abnormal embryos from IVF were cultured in serum-free Minimum Essential Medium (MEM).
  • Blastocysts were cultured up to day 14 post-fertilization.
  • The effect of human cord serum, insulin+transferrin+selenium (ITS), platelet-derived growth factor (PDGF), and 8-bromo-cAMP on CG secretion was assessed.

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Main Results:

  • Human cord serum (1%) was a potent stimulant for CG production.
  • ITS, PDGF, and 8-bromo-cAMP also stimulated CG secretion in serum-free MEM.
  • These stimulants were effective in day 7-8 blastocysts, indicating multiple factors can induce CG production.

Conclusions:

  • Multiple factors can induce chorionic gonadotrophin (CG) production by early blastocysts.
  • Preimplantation blastocysts are postulated to express receptors for insulin, transferrin, and PDGF.
  • These findings contribute to understanding early embryonic signaling and development.