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Related Experiment Videos

[BHV-1 intradermal test in cattle]

M Metzner1, W Hofmann, H W Lessmann

  • 1Klinik für Klauentiere, Fortpflanzung und Haltungshygiene, Freien Universität Berlin.

Tierarztliche Praxis
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

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This study evaluated an intradermal test for diagnosing Bovine Herpesvirus 1 (BHV 1) infection in cattle. The test showed variable results and could induce seroconversion in some animals.

Area of Science:

  • Veterinary Immunology
  • Infectious Disease Diagnostics
  • Bovine Virology

Context:

  • Bovine Herpesvirus 1 (BHV 1) causes significant economic losses in cattle farming.
  • Accurate diagnostic tools are crucial for managing BHV 1 outbreaks.
  • Current diagnostic methods have limitations in sensitivity and specificity.

Purpose:

  • To investigate the diagnostic performance of an intradermal test for BHV 1 infection.
  • To assess the reliability and potential side effects of the intradermal test.
  • To compare intradermal test results with serological detection of BHV 1 antibodies.

Summary:

  • An intradermal test for BHV 1 diagnosis was evaluated in 221 cattle.
  • Test results varied based on reading time (48 vs. 72 hours) in 16% of cases.

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  • Fifteen percent of cattle over 9 months old, despite having detectable antibodies (ELISA), showed no reaction to the intradermal test.
  • Intradermal antigen injection induced seroconversion in seronegative animals, with antibodies detectable by ELISA for 3-6 weeks.

    • Impact:

    • The intradermal test's diagnostic utility is limited by variable results and potential for false negatives in older animals.
    • The test can induce an immune response, complicating interpretation in previously seronegative cattle.
    • Further research is needed to refine the intradermal test or explore alternative diagnostic strategies for BHV 1.