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Updated: Jun 11, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
Isolation of a repeated sequence from the genome of Bordetella parapertussis with characteristics of an insertion
A van der Zee1, C Agterberg, M van Agterveld
1Laboratory of Medical Microbiology, St. Elisabeth Hospital, Tillburg, The Netherlands.
Abstract:
A repeated DNA sequence in the genome of Bordetella parapertussis was identified by means of heteroduplex formation and S1 nuclease treatment. Cloning and sequencing of the repeated sequence, revealed that it comprised 1306 basepairs, contained inverted repeats at its termini, and two large open reading frames. This insertion sequence element was designated IS1001, and was found in about 20 copies in 10 B. parapertussis strains analyzed. Identical hybridization patterns were observed in B. parapertussis when IS1001 was used as a probe. B. pertussis strains did not hybridize to IS1001.
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