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Related Experiment Videos

Functional analysis of human foamy virus accessory reading frames

G Baunach1, B Maurer, H Hahn

  • 1Institut für Virologie und Immunbiologie der Universität, Würzburg, Germany.

Journal of Virology
|September 1, 1993
PubMed
Summary

The bel-1 gene is essential for human foamy virus (HFV) replication. Other accessory genes are not required for HFV replication or gag protein expression, unlike in other complex retroviruses.

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Area of Science:

  • Retroviruses
  • Molecular Virology

Background:

  • Foamy viruses are complex retroviruses with unique genome structures.
  • The human foamy virus (HFV) genome contains three bel genes (open reading frames) in its 3' region.
  • Alternative splicing of bel genes can produce up to six different proteins.

Purpose of the Study:

  • To investigate the role of bel genes in HFV replication in vitro.
  • To determine the necessity of accessory bel gene products for viral replication and protein expression.

Main Methods:

  • Construction of single and combined bel gene mutants from an infectious HFV molecular clone.
  • Analysis of viral replication competence and infectious virus titers.
  • Expression studies using HFV proviruses under a heterologous promoter (simian virus 40).

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Main Results:

  • A mutant lacking the bel-1 transactivator was replication incompetent.
  • All other bel gene mutants replicated efficiently, producing comparable titers of infectious virus.
  • Accessory bel gene products were not required for structural (gag) protein expression when HFV was under heterologous promoter control.

Conclusions:

  • The bel-1 gene is crucial for HFV replication.
  • Accessory bel genes are dispensable for HFV replication and gag protein expression.
  • HFV lacks a posttranscriptional regulatory protein found in other complex retroviruses.