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Multiple human papillomavirus type 16 glucocorticoid response elements functional for transformation, transient
1Division of Basic Medical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Journal of Virology
|September 1, 1993
Summary
Human papillomavirus type 16 (HPV-16) requires specific glucocorticoid response elements (GREs) for hormone-induced cell transformation. The glucocorticoid receptor protein binds to these identified HPV-16 GREs, crucial for dexamethasone
Area of Science:
- Molecular biology
- Virology
- Endocrinology
Background:
- Human papillomavirus type 16 (HPV-16) can transform cells with steroid hormones.
- Glucocorticoid response elements (GREs) are involved in HPV-16 mediated transformation.
Purpose of the Study:
- To investigate the role of specific GREs in HPV-16-induced cell transformation.
- To understand the interaction between the glucocorticoid receptor and HPV-16 GREs.
Main Methods:
- Site-directed mutagenesis of identified GREs in HPV-16.
- Transient transfection assays in cervical HeLa cells.
- In vitro protein-DNA binding assays.
Main Results:
- All three identified GREs in HPV-16 are functional in mediating dexamethasone-induced transformation.
- Transient assays confirmed the functional significance of individual GREs.
- The glucocorticoid receptor specifically binds to the newly identified HPV-16 GREs.
Conclusions:
- The study elucidates the critical role of three specific GREs in HPV-16's transformation capabilities.
- Dexamethasone-induced transformation is mediated by the glucocorticoid receptor binding to these GREs.
- These findings contribute to understanding HPV-16 oncogenesis and potential therapeutic targets.